Proliferation and neural stem cell potential of diencephalic astrocytes revealed by genome-wide expression is SMAd4 dependent – scRNA-seq I Version 1
External LinkDiencephalic astrocytes from 3 months old Aldh1l1-eGFP mice were ACSA2 MACS sorted and processed using Single Cell 3’ Reagent Kits V2 from 10x Genomics (scRNA-seq).
Export PNG
Download
Creators and Submitter
Views: 52
Created: 14th Oct 2024 at 13:40
TagsThis item has not yet been tagged.
AttributionsNone
Version History
Version 1 (earliest) Created 14th Oct 2024 at 13:40 by Aditi Methi
No revision comments
Related items
Projects: SyNergy - Published Datasets
Institutions: Klinikum der Universität München
Public web page: Not specified
Submitter: Rainer Malik
Studies: A genome-wide in vivo CRISPR screen identifies essential regulators of T..., Adult neural stem cell activation in mice is regulated by the day/night ..., Direct neuronal reprogramming of NDUFS4 patient cells identifies the unf..., Early adaptive immune activation detected in monozygotic twins with prod..., Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the..., Histone Deacetylase 9 Activates IKK to Regulate Atherosclerotic Plaque V..., Innate Immune Pathways Promote Oligodendrocyte Progenitor Cell Recruitme..., Innate immune memory after brain injury drives inflammatory cardiac dysf..., Microglia in white matter aging, Molecular diversity of diencephalic astrocytes reveals adult astrogenesi..., Multiomic ALS signatures highlight subclusters and sex differences sugge..., Myelin degeneration in leucodystrophies, Oligodendrocytes in AD models, Oligodendrocytes in white matter aging, Parkinson's disease motor symptoms rescue by CRISPRa‐reprogramming astro..., Phagocyte-mediated synapse removal in cortical neuroinflammation is prom..., Shared inflammatory glial cell signature after stab wound injury, reveal..., Skin and gut imprinted helper T cell subsets exhibit distinct functional..., Spatial Transcriptomics-correlated Electron Microscopy maps transcriptio..., Spatial centrosome proteome of human neural cells uncovers disease-relev..., T cells modulate the microglial response to brain ischemia
Assays: Expression profiling: Bulk RNA-seq (human), Expression profiling: Bulk RNA-seq (human), Expression profiling: Bulk RNA-seq (human), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (rat), Expression profiling: MERFISH Spatial Transcriptomics (mouse), Expression profiling: Microarray (zebrafish), Expression profiling: Spatial Transcriptomics (mouse), Expression profiling: Spatial Transcriptomics correlated Electron Micros..., Expression profiling: scRNA-seq (human), Expression profiling: scRNA-seq (human), Expression profiling: scRNA-seq (human), Expression profiling: scRNA-seq (human) (Day 20), Expression profiling: scRNA-seq (human) (Day 5), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: small RNA-seq (human), Expression profiling: small RNA-seq (mouse), Genome binding/occupancy profiling: Bulk ATAC-seq (mouse), Genome binding/occupancy profiling: Bulk ATAC-seq (mouse), Genome binding/occupancy profiling: CUT&Tag sequencing (mouse), Genome binding/occupancy profiling: snATAC-seq (mouse), Genome wide (GW) and validation CRISPR screens (rat)
Astrocytes regulate brain‐wide functions and also show region‐specific differences, but little is known about how general and region‐specific functions are aligned at the single‐cell level. To explore this, we isolated adult mouse diencephalic astrocytes by ACSA‐2‐mediated magnetic‐activated cell sorting (MACS). Single‐cell RNA‐seq revealed 7 gene expression clusters of astrocytes, with 4 forming a supercluster. Within the supercluster, cells differed by gene expression related to ion homeostasis ...
Submitter: Aditi Methi
Investigation: Transcriptomics
Assays: Expression profiling: Bulk RNA-seq (mouse), Expression profiling: scRNA-seq (mouse)
Submitter: Aditi Methi
Assay type: Transcriptomics
Technology type: Sequencing
Investigation: Transcriptomics
