Expression profiling: scRNA-seq (mouse)
SEEK ID: http://localhost:3000/assays/82
Experimental assay
Projects: SyNergy - Published Datasets
Investigation: Transcriptomics
Study: Molecular diversity of diencephalic astrocytes reveals adult astrogenesis regulated by Smad4
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Assay type: Transcriptomics
Technology type: Sequencing
Organisms: Mus musculus
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Created: 14th Oct 2024 at 13:39
Last updated: 14th Oct 2024 at 15:46
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Projects: SyNergy - Published Datasets
Institutions: Klinikum der Universität München
Public web page: Not specified
Submitter: Rainer Malik
Studies: A genome-wide in vivo CRISPR screen identifies essential regulators of T..., Adult neural stem cell activation in mice is regulated by the day/night ..., Direct neuronal reprogramming of NDUFS4 patient cells identifies the unf..., Early adaptive immune activation detected in monozygotic twins with prod..., Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the..., Histone Deacetylase 9 Activates IKK to Regulate Atherosclerotic Plaque V..., Innate Immune Pathways Promote Oligodendrocyte Progenitor Cell Recruitme..., Innate immune memory after brain injury drives inflammatory cardiac dysf..., Microglia in white matter aging, Molecular diversity of diencephalic astrocytes reveals adult astrogenesi..., Multiomic ALS signatures highlight subclusters and sex differences sugge..., Myelin degeneration in leucodystrophies, Oligodendrocytes in AD models, Oligodendrocytes in white matter aging, Parkinson's disease motor symptoms rescue by CRISPRa‐reprogramming astro..., Phagocyte-mediated synapse removal in cortical neuroinflammation is prom..., Shared inflammatory glial cell signature after stab wound injury, reveal..., Skin and gut imprinted helper T cell subsets exhibit distinct functional..., Spatial Transcriptomics-correlated Electron Microscopy maps transcriptio..., Spatial centrosome proteome of human neural cells uncovers disease-relev..., T cells modulate the microglial response to brain ischemia
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Astrocytes regulate brain‐wide functions and also show region‐specific differences, but little is known about how general and region‐specific functions are aligned at the single‐cell level. To explore this, we isolated adult mouse diencephalic astrocytes by ACSA‐2‐mediated magnetic‐activated cell sorting (MACS). Single‐cell RNA‐seq revealed 7 gene expression clusters of astrocytes, with 4 forming a supercluster. Within the supercluster, cells differed by gene expression related to ion homeostasis ...
Submitter: Aditi Methi
Investigation: Transcriptomics
Assays: Expression profiling: Bulk RNA-seq (mouse), Expression profiling: scRNA-seq (mouse)
Diencephalic astrocytes from 3 months old Aldh1l1-eGFP mice were ACSA2 MACS sorted and processed using Single Cell Reagent Kits V2 from 10x Genomics (scRNA-seq).
External LinkDiencephalic astrocytes from 3 months old Aldh1l1-eGFP mice were ACSA2 MACS sorted and processed using Single Cell 3’ Reagent Kits V2 from 10x Genomics (scRNA-seq).
