Expression profiling by high throughput sequencing
SEEK ID: http://localhost:3000/assays/5
Experimental assay
Projects: SyNergy - published datasets
Investigation: Transcriptomics
Study: Microglia in white matter aging
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Assay type: Experimental Assay Type
Technology type: Technology Type
Organisms: No organisms
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Creators and SubmitterViews: 16
Created: 27th Jun 2024 at 11:55
Last updated: 27th Jun 2024 at 11:57
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Projects: SyNergy - published datasets
Institutions: Klinikum der Universität München
Public web page: Not specified
Submitter: Rainer Malik
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Submitter: Rainer Malik
Investigation: Transcriptomics
Grey and white matter (corpus callosum) were dissected from the brain of 4 months and 21 months old wild type mice, single cell suspension was isolated using Neural Tissue Dissection kit (myelin was removed on percoll gradient) then microglia cells were isolated using CD11b microbeads by Magnetic separation with MS columns (MACS technology).The cell pellets are resuspended in the RLT buffer (100ul-150ul), snap frozen in liquid nitrogen and stored in -80 freezer. RNA was extracted and transcriptome ...
External LinkAging results in both grey and white matter degeneration, but the specific microglial responses are unknown. Using single-cell RNA sequencing from white and grey matter separately, we identified white matter associated microglia (WAM), which share parts of the disease-associated microglia (DAM) gene signature and are characterized by the activation of genes implicated in phagocytic activity and lipid metabolism. WAM depend on triggering receptor expressed on myeloid cells 2 (TREM2) signaling and ...
