Strain details
Name | Provider name | Provider's strain ID | Genotypes | Phenotypes | Synonym | Comments | Based on |
---|
Related items
Public web page: Not specified
Organisms: Mus musculus, Rattus norvegicus, Homo sapiens, Macaca mulatta, Sus scrofa, Danio rerio
Submitter: Rainer Malik
Assay type: CRISPR Screen
Technology type: Technology Type
Investigation: Transcriptomics
Organisms: Rattus norvegicus
SOPs: No SOPs
Data files: Genome wide (GW) and validation CRISPR screens ...
Snapshots: No snapshots
The neurons were lysed direct on the plate in RIPA lysis buffer using a cell scraper. The lysate was transferred into a fresh Eppendorf tube and undissolved material was removed by centrifugation for 5 min at 20,000 g and 4°C. A protein assay was performed and 15 µg of protein were subjected to proteolytic digestion with the SP3 protocol. Proteins were reduced by addition of 9 µL of 200 mM dithiothreitol (Biozol, Germany) in 50 mM ammonium bicarbonate and incubation for 30 min at 37°C. Cysteine ...
Submitter: Rainer Malik
Assay type: Proteomics
Technology type: Technology Type
Investigation: Proteomics
Organisms: Rattus norvegicus
SOPs: No SOPs
Data files: Growth factor-mediated regulation of neuronal e..., Perilous Sites: High basic Fibroblast Growth Fa...
Snapshots: No snapshots
Submitter: Aditi Methi
Assay type: Transcriptomics
Technology type: Sequencing
Investigation: Transcriptomics
Organisms: Rattus norvegicus
SOPs: No SOPs
Data files: Effect of Grk2 (spleen, rat) or Ets1 (spleen an...
Snapshots: No snapshots