Microglia in white matter aging
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Created: 27th Jun 2024 at 11:51
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Projects: SyNergy - Published Datasets
Institutions: LMU
Projects: SyNergy - Published Datasets, HTRA1
Institutions: Klinikum der Universität München
https://orcid.org/0000-0001-5329-192X
Public web page: Not specified
Submitter: Rainer Malik
Studies: A genome-wide in vivo CRISPR screen identifies essential regulators of T..., Adult neural stem cell activation in mice is regulated by the day/night ..., Direct neuronal reprogramming of NDUFS4 patient cells identifies the unf..., Early adaptive immune activation detected in monozygotic twins with prod..., Heterogeneity of neurons reprogrammed from spinal cord astrocytes by the..., Histone Deacetylase 9 Activates IKK to Regulate Atherosclerotic Plaque V..., Innate Immune Pathways Promote Oligodendrocyte Progenitor Cell Recruitme..., Innate immune memory after brain injury drives inflammatory cardiac dysf..., Microglia in white matter aging, Molecular diversity of diencephalic astrocytes reveals adult astrogenesi..., Multiomic ALS signatures highlight subclusters and sex differences sugge..., Myelin degeneration in leucodystrophies, Oligodendrocytes in AD models, Oligodendrocytes in white matter aging, Parkinson's disease motor symptoms rescue by CRISPRa‐reprogramming astro..., Phagocyte-mediated synapse removal in cortical neuroinflammation is prom..., Shared inflammatory glial cell signature after stab wound injury, reveal..., Skin and gut imprinted helper T cell subsets exhibit distinct functional..., Spatial Transcriptomics-correlated Electron Microscopy maps transcriptio..., Spatial centrosome proteome of human neural cells uncovers disease-relev..., T cells modulate the microglial response to brain ischemia
Assays: Expression profiling: Bulk RNA-seq (human), Expression profiling: Bulk RNA-seq (human), Expression profiling: Bulk RNA-seq (human), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (mouse), Expression profiling: Bulk RNA-seq (rat), Expression profiling: MERFISH Spatial Transcriptomics (mouse), Expression profiling: Microarray (zebrafish), Expression profiling: Spatial Transcriptomics (mouse), Expression profiling: Spatial Transcriptomics correlated Electron Micros..., Expression profiling: scRNA-seq (human), Expression profiling: scRNA-seq (human), Expression profiling: scRNA-seq (human), Expression profiling: scRNA-seq (human) (Day 20), Expression profiling: scRNA-seq (human) (Day 5), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: scRNA-seq (mouse), Expression profiling: small RNA-seq (human), Expression profiling: small RNA-seq (mouse), Genome binding/occupancy profiling: Bulk ATAC-seq (mouse), Genome binding/occupancy profiling: Bulk ATAC-seq (mouse), Genome binding/occupancy profiling: CUT&Tag sequencing (mouse), Genome binding/occupancy profiling: snATAC-seq (mouse), Genome wide (GW) and validation CRISPR screens (rat)
Submitter: Rainer Malik
Assay type: Transcriptomics
Technology type: Sequencing
Investigation: Transcriptomics
Submitter: Aditi Methi
Assay type: Transcriptomics
Technology type: Sequencing
Investigation: Transcriptomics
Study of microglia in aging and in a tissue specific manner (white and grey matter) using Smart-seq2 and 10x scRNA-seq technologies. We also analysed, 5xFAD mice (AD model) with Smart-seq2, and Apoe-KO with 10x transcriptomics. Additionally we studied the impact of Actinomycin-D during tissue dissociation on gene expression in microglia using Smart-seq2.
Creator: Rainer Malik
Submitter: Rainer Malik
External LinkGrey and white matter (corpus callosum) were dissected from the brain of 4 months and 21 months old wild type mice, single cell suspension was isolated using Neural Tissue Dissection kit (myelin was removed on percoll gradient) then microglia cells were isolated using CD11b microbeads by Magnetic separation with MS columns (MACS technology).The cell pellets are resuspended in the RLT buffer (100ul-150ul), snap frozen in liquid nitrogen and stored in -80 freezer. RNA was extracted and transcriptome ...
Creator: Mikael Simons
Submitter: Rainer Malik
Abstract (Expand)
Authors: S. Safaiyan, S. Besson-Girard, T. Kaya, L. Cantuti-Castelvetri, L. Liu, H. Ji, M. Schifferer, G. Gouna, F. Usifo, N. Kannaiyan, D. Fitzner, X. Xiang, M. J. Rossner, M. Brendel, O. Gokce, M. Simons
Date Published: 7th Apr 2021
Publication Type: Journal
