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Organisms: Mus musculus, Rattus norvegicus, Homo sapiens, Macaca mulatta, Sus scrofa
Mitochondria account for essential cellular pathways, from ATP production to nucleotide metabolism, and their deficits lead to neurological disorders and contribute to the onset of age-related diseases. Direct neuronal reprogramming aims at replacing neurons lost in such conditions, but almost nothing is known about the impact of mitochondrial dysfunction in human cell direct reprogramming. Here we explore the defects and how to improve the neuronal reprogramming of iPSC-derived astrocytes carrying ...
Submitter: Rainer Malik
Investigation: Transcriptomics
Assays: Bulk RNA-seq, scRNA-seq 5 day, scRNA-seq day 20
Snapshots: No snapshots
human IPSCs from control or patients with NDUFS4 mutations were differentiated into proliferating and non-proliferating astrocytes. Three control and 3 patients lines were analyzed. Per each line, 3 biological replicates were collected at 3 stages: IPSC, proliferating astrocyte and non-proliferating astrocytes.
Submitter: Rainer Malik
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Transcriptomics
Organisms: No organisms
SOPs: No SOPs
Data files: Unfolded protein Response is a major hurdle in ...
Snapshots: No snapshots
pAstros were transduced with control or Ngn2-expressing retrovirus. 2DPT, media was replaced with differentiation media in absence of presence of AMG; 5DPT, DsRed-positive cells were sorted, barcoded with CMOs and analyzed by scRNAseq via 10XGenoics platform
Submitter: Rainer Malik
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Transcriptomics
Organisms: Homo sapiens
SOPs: No SOPs
Data files: Unfolded protein Response is a major hurdle in ...
Snapshots: No snapshots
pAstros were transduced with control or Ngn2-expressing retrovirus. 2DPT, media was replaced with differentiation media in absence of presence of AMG; 20DPT, DsRed-positive cells were sorted, barcoded with CMOs and analyzed by scRNAseq via 10XGenoics platform
Submitter: Rainer Malik
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Transcriptomics
Organisms: Homo sapiens
SOPs: No SOPs
Data files: Unfolded protein Response is a major hurdle in ...
Snapshots: No snapshots
Mitochondria account for essential cellular pathways, from ATP production to nucleotide metabolism, and their deficits lead to neurological disorders and contribute to the onset of age-related diseases. Direct neuronal reprogramming aims at replacing neurons lost in such conditions, but almost nothing is known about the impact of mitochondrial dysfunction in human cell direct reprogramming. Here we explore the defects and how to improve the neuronal reprogramming of iPSC-derived astrocytes carrying ...
Creators: Rainer Malik, Magdalena Götz
Submitter: Rainer Malik
Investigations: Transcriptomics
Studies: Direct neuronal reprogramming of NDUFS4 patient...
Assays: scRNA-seq day 20
Mitochondria account for essential cellular pathways, from ATP production to nucleotide metabolism, and their deficits lead to neurological disorders and contribute to the onset of age-related diseases. Direct neuronal reprogramming aims at replacing neurons lost in such conditions, but almost nothing is known about the impact of mitochondrial dysfunction in human cell direct reprogramming. Here we explore the defects and how to improve the neuronal reprogramming of iPSC-derived astrocytes carrying ...
Creators: Rainer Malik, Magdalena Götz
Submitter: Rainer Malik
Investigations: Transcriptomics
Studies: Direct neuronal reprogramming of NDUFS4 patient...
Assays: scRNA-seq 5 day
Mitochondria account for essential cellular pathways, from ATP production to nucleotide metabolism, and their deficits lead to neurological disorders and contribute to the onset of age-related diseases. Direct neuronal reprogramming aims at replacing neurons lost in such conditions, but almost nothing is known about the impact of mitochondrial dysfunction in human cell direct reprogramming. Here we explore the defects and how to improve the neuronal reprogramming of iPSC-derived astrocytes carrying ...
Creators: Rainer Malik, Magdalena Götz
Submitter: Rainer Malik
Investigations: Transcriptomics
Studies: Direct neuronal reprogramming of NDUFS4 patient...
Assays: Bulk RNA-seq
Abstract (Expand)
Authors: G. Sonsalla, A. B. Malpartida, T. Riedemann, M. Gusic, E. Rusha, G. Bulli, S. Najas, A. Janjic, B. A. Hersbach, P. Smialowski, M. Drukker, W. Enard, J. H. M. Prehn, H. Prokisch, M. Gotz, G. Masserdotti
Date Published: 3rd Apr 2024
Publication Type: Journal
PubMed ID: 38266647
Citation: Neuron. 2024 Apr 3;112(7):1117-1132.e9. doi: 10.1016/j.neuron.2023.12.020. Epub 2024 Jan 23.