Proteomics of cerebrospinal fluid (CSF) of acitretin treated patients Version 1
External LinkAcitretin has been shown to increase ADAM10 expression. In a phase II clinical trial, Alzheimer's disease patients were treated with acitretin or placebo to increase ADAM10 levels to counteract amyloid beta production. Analysis of the cerebrospinal fluid of from 18 AD patients treated with the synthetic retinoid acitretin or placebo were analyzed by label-free quantitative LC-MS/MS analysis.
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Created: 8th Jul 2024 at 09:56
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Version 1 (earliest) Created 8th Jul 2024 at 09:56 by Rainer Malik
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Projects: SyNergy - published datasets
Institutions: DZNE
Projects: SyNergy - published datasets
Institutions: Klinikum der Universität München
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Institutions: DZNE
Public web page: Not specified
Submitter: Rainer Malik
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Nine patients per group were treated with either acitretin or vehicle control. Cerebrospinal fluid was collected before (baseline value) and after treatment. A volume of 5 µL of CSF per sample was subjected to proteolytic digestion in 50 mM ammonium bicarbonate with 0.1% sodium deoxycholate (Sigma Aldrich, Germany) as previously described (Pigoni et al., 2016). Briefly, protein disulfide bonds were reduced with dithiothreitol and sulfhydryl residues were alkylated using iodoacetamide. Proteins ...
Submitter: Rainer Malik
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Proteomics
Abstract (Expand)
Authors: T. Brummer, S. A. Muller, F. Pan-Montojo, F. Yoshida, A. Fellgiebel, T. Tomita, K. Endres, S. F. Lichtenthaler
Date Published: 6th Mar 2019
Publication Type: Journal
