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The glial environment influences neurological disease progression, yet much of our knowledge still relies on preclinical animal studies, especially regarding astrocyte heterogeneity. In murine models of traumatic brain injury, beneficial functions of proliferating reactive astrocytes on disease outcome have been unraveled, but little is known regarding if and when they are present in human brain pathology. Here we examined a broad spectrum of pathologies with and without intracerebral hemorrhage and found a striking correlation between lesions involving blood-brain barrier rupture and astrocyte proliferation that was further corroborated in an assay probing for neural stem cell potential. Most importantly, proteomic analysis unraveled a crucial signaling pathway regulating this astrocyte plasticity with GALECTIN3 as a novel marker for proliferating astrocytes and the GALECTIN3-binding protein LGALS3BP as a functional hub mediating astrocyte proliferation and neurosphere formation. Taken together, this work identifies a therapeutically relevant astrocyte response and their molecular regulators in different pathologies affecting the human cerebral cortex.
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Created: 15th Oct 2024 at 13:02
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Projects: Published Datasets, Unpublished Datasets
Institutions: Helmholtz Munich, LMU
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Projects: Published Datasets, Unpublished Datasets
Institutions: LMU Klinikum
Research Data Manager
Projects: Published Datasets, Unpublished Datasets
Institutions: Helmholtz Munich
Please visit the 'Related items' tab within the profile page to explore associated studies in more detail.
This project serves as a centralized repository for omics datasets published by research groups within the SyNergy Cluster. It encompasses investigations such as proteomics and transcriptomics, which are further divided into individual studies led by SyNergy members. Each study is linked to relevant publications, assays and data files (with links to external repositories).
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Public web page: Not specified
Organisms: Mus musculus, Rattus norvegicus, Homo sapiens, Macaca mulatta, Sus scrofa, Danio rerio
Submitter: Rainer Malik
Studies: A TBK1 variant causes autophagolysosomal and motoneuron pathology withou..., A ubiquitin-specific, proximity-based labeling approach for the identifi..., ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid dr..., ADAM10-Mediated Ectodomain Shedding Is an Essential Driver of Podocyte D..., ALS-linked loss of Cyclin-F function affects HSP90, AMPK, a Regulator of Metabolism and Autophagy, Is Activated by Lysosomal..., ATG4 family proteins drive phagophore growth independently of the LC3/GA..., An optimized quantitative proteomics method establishes the cell type-re..., Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mit..., Autophagosome content profiling using proximity biotinylation proteomics..., Autophagy acts through TRAF3 and RELB to regulate gene expression via an..., Basic Fibroblast Growth Factor 2-Induced Proteome Changes Endorse Lewy B..., Beneficial Effect of ACI-24 Vaccination on Aβ Plaque Pathology and Micro..., Brain injury environment critically influences the connectivity of trans..., C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin c..., CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boos..., Cell-type-specific profiling of brain mitochondria reveals functional an..., Cellular depletion of major cathepsin proteases reveals their concerted ..., Deciphering sources of PET signals in the tumor microenvironment of glio..., Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regula..., Development of a Proteomic Workflow for the Identification of Heparan Su..., Distinct molecular profiles of skull bone marrow in health and neurologi..., Excessive local host-graft connectivity in aging and amyloid-loaded brain, Experimental evidence for temporal uncoupling of brain Aβ deposition and..., Fibrillar Aβ triggers microglial proteome alterations and dysfunction in..., Filling the Gaps – A Call for Comprehensive Analysis of Extracellular Ma..., IKKβ binds NLRP3 providing a shortcut to inflammasome activation for rap..., Injury-specific factors in the cerebrospinal fluid regulate astrocyte pl..., Lipid and protein content profiling of isolated native autophagic vesicles, Loss of CLN3 in microglia leads to impaired lipid metabolism and myelin ..., Loss of NPC1 enhances phagocytic uptake and impairs lipid trafficking in..., Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH, Lysosomal targeting of the ABC transporter TAPL is determined by membran..., Mapping autophagosome contents identifies interleukin-7 receptor-alpha a..., Met/HGFR triggers detrimental reactive microglia in TBI, MicroRNAs from extracellular vesicles as a signature for Parkinson's dis..., Multi-omics profiling identifies a deregulated FUS-MAP1B axis in ALS/FTD..., Multiomic ALS signatures highlight subclusters and sex differences sugge..., Neuronal differentiation of LUHMES cells induces substantial changes of ..., Nonvesicular lipid transfer drives myelin growth in the central nervous ..., NrCAM is a marker for substrate-selective activation of ADAM10 in Alzhei..., Pro-inflammatory activation following demyelination is required for myel..., Proteomic Characterization of Ubiquitin Carboxyl-Terminal Hydrolase 19 D..., Proteomic and lipidomic profiling of demyelinating lesions identifies fa..., Proteomic profiling in cerebral amyloid angiopathy reveals an overlap wi..., Proteomics of mouse brain endothelium uncovers dysregulation of vesicula..., Rational correction of pathogenic conformational defects in HTRA1, Reactivated endogenous retroviruses promote protein aggregate spreading, Signal peptide peptidase-like 2c impairs vesicular transport and cleaves..., Signatures of glial activity can be detected in the CSF proteome, Spatial centrosome proteome of human neural cells uncovers disease-relev..., Spatial proteomics in three-dimensional intact specimens, Spatial proteomics reveals secretory pathway disturbances caused by neur..., Systematically defining selective autophagy receptor-specific cargo usin..., Targeting the TCA cycle can ameliorate widespread axonal energy deficien..., The Alzheimer's disease-linked protease BACE1 modulates neuronal IL-6 si..., The Alzheimer's disease-linked protease BACE2 cleaves VEGFR3 and modulat..., The COP9 signalosome reduces neuroinflammation and attenuates ischemic n..., The Hippo network kinase STK38 contributes to protein homeostasis by inh..., The intramembrane protease SPPL2c promotes male germ cell development by..., The pseudoprotease iRhom1 controls ectodomain shedding of membrane prote..., The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor com..., The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in respon..., Trnp1 organizes diverse nuclear membrane-less compartments in neural ste..., Ubiquitin profiling of lysophagy identifies actin stabilizer CNN2 as a t...
Assays: Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Bottom-up proteomics (human), Bottom-up proteomics (mouse), Bottom-up proteomics (mouse), Bottom-up proteomics (mouse), Bottom-up proteomics (mouse), Gel-based experiment (human), Phosphoproteomics / Bottom-up proteomics (mouse), Proximity-proteomics-based autophagosome content profiling (human), SWATH MS (human), SWATH MS (human, mouse), SWATH MS (mouse), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human, mouse), Shotgun proteomics (human, mouse), Shotgun proteomics (human, mouse), Shotgun proteomics (human, mouse), Shotgun proteomics (macaque), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (rat), Untargeted Proteomics (mouse)
Snapshots: Snapshot 1
Total protein content of CSF samples was measured by Bradford assay (BioRad, Feldkirchen, Germany)and 10μg per sample were proteolysed by the commercially available in-StageTip-NHS kit (PreOmics GmbH, Martinsried, Germany) according to the manufacturer's protocol. Briefly, CSF was reduced and alkylated and incubated for 3 hrs at 37°C with Lys-C and trypsin. Resulting peptides were dried for short term storage at -80°C. Prior to measurement, peptides were resuspended in 2% acetonitrile and 0.5% ...
Submitter: Aditi Methi
Assay type: Proteomics
Technology type: Technology Type
Investigation: Proteomics (Published)
Organisms: Homo sapiens
SOPs: No SOPs
Data files: Injury-specific factors in the cerebrospinal fl...
Snapshots: No snapshots
Creators: None
Submitter: Aditi Methi
Investigations: Proteomics (Published)
Studies: Injury-specific factors in the cerebrospinal fl...
Assays: Shotgun proteomics (human)
Abstract (Expand)
Authors: Swetlana Sirko, Christian Schichor, Patrizia Della Vecchia, Fabian Metzger, Giovanna Sonsalla, Tatiana Simon, Martina Bürkle, Sofia Kalpazidou, Jovica Ninkovic, Giacomo Masserdotti, Jean-Frederic Sauniere, Valentina Iacobelli, Stefano Iacobelli, Claire Delbridge, Stefanie M Hauck, Jörg-Christian Tonn, Magdalena Götz
Date Published: 8th Dec 2023
Publication Type: Journal