Affinity purification coupled with mass spectrometry proteomics (human)
Samples were essentially prepared as described in Zellner et al. Mol Cell 2021 (DOI: 10.1016/j.molcel.2021.01.009)
SEEK ID: http://lmmeisd-2.srv.mwn.de/assays/57
Experimental assay
Projects: Published Datasets
Investigation: Proteomics (Published)
Assay position:
Assay type: Proteomics
Technology type: Technology Type
Organisms: Homo sapiens
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Created: 9th Jul 2024 at 13:39
Last updated: 15th Oct 2024 at 12:26
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Projects: Published Datasets, Unpublished Datasets
Institutions: LMU Klinikum
Research Data Steward
This project serves as a centralized repository for omics datasets published by research groups within the SyNergy Cluster. It encompasses investigations such as proteomics and transcriptomics, which are further divided into individual studies led by SyNergy members. Each study is linked to relevant publications, assays and data files (with links to external repositories).
To explore investigations and their associated studies in more detail, please visit the 'Related items' tab on the Project ...
Public web page: Not specified
Submitter: Rainer Malik
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The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry that is suitable to specifically map the content of autophagosomes and potentially other transient vesicles without the need of subcellular fractionation. By combining live-cell biotinylation with proteinase K digestion of cell homogenates, proteins enriched ...
Submitter: Rainer Malik
Investigation: Proteomics (Published)
Assays: Affinity purification coupled with mass spectrometry proteomics (human)
The ascorbate peroxidase APEX2 is commonly used to study the neighborhood of a protein of interest by proximity-dependent biotinylation. Here, we describe a protocol for sample processing compatible with immunoblotting and mass spectrometry that is suitable to specifically map the content of autophagosomes and potentially other transient vesicles without the need of subcellular fractionation. By combining live-cell biotinylation with proteinase K digestion of cell homogenates, proteins enriched ...
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