Proximity-proteomics-based autophagosome content profiling (human)

Proximity-proteomics-based autophagosome content profiling to identify a role for LC3C in maintaining basal mitochondrial homeostasis. Selected mitochondrial proteins, including MTX1, were targeted by LC3C and p62 through a piecemeal mitophagy pathway. SILAC cells biotinylated using APEX proximity labeling. Cell lysates treated with protease. RIPA soluble and insoluble fractions were subjected to Streptavidin pulldown followed by in gel digestion. 4 lanes were cut from one sample.

SEEK ID: http://localhost:3000/assays/91

Experimental assay

Aditi Methi

Projects: Published Datasets

Investigation: Proteomics (Published)

Study: Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway

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Assay type: Proteomics

Technology type: Technology Type

Organisms: Homo sapiens

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Created: 15th Oct 2024 at 12:55

Last updated: 15th Oct 2024 at 12:56

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