Proteomic signature of NPC1 KO microglia from conditional KO mice Version 1
External LinkNiemann-Pick type C (NPC) disease is a rare neurodegenerative disorder mainly caused by autosomal recessive mutations in Npc1 which result in abnormal late endosomal/lysosomal lipid storage. Although microgliosis is one of the prominent pathological features, consequences of NPC1 loss on microglial function and disease outcome remain largely unknown. Here, we provide an in-depth characterization of microglial proteomic signatures and phenotypes in an NPC1-deficient (Npc1-/-) murine model. We demonstrate that microglial defects, including enhanced phagocytosis and impaired lipid trafficking, occur early in the NPC pathological cascade and precede neuronal death. Compromised microglial function during Npc1-/- mouse development is reflected by enhanced synaptic pruning and deficient turnover of myelin. Accumulation of the undigested myelin occurs mainly within multi-vesicular bodies (MVBs) of Npc1-/- microglia and not within lysosomes. This is in agreement with the impairments in recycling of myelin into lipid droplets. Macrophages of NPC patients displayed similar molecular and functional alterations as murine Npc1-/- microglia, strengthening the role of NPC1 in immune homeostasis. Generated ex vivo assays using patient macrophages are novel promising clinical tools to monitor the progression and therapeutic efficacy in NPC patients.
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Created: 8th Jul 2024 at 08:57
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Version 1 (earliest) Created 8th Jul 2024 at 08:57 by Rainer Malik
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Institutions: LMU Klinikum
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Submitter: Rainer Malik
Studies: A TBK1 variant causes autophagolysosomal and motoneuron pathology withou..., A ubiquitin-specific, proximity-based labeling approach for the identifi..., ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid dr..., ADAM10-Mediated Ectodomain Shedding Is an Essential Driver of Podocyte D..., ALS-linked loss of Cyclin-F function affects HSP90, AMPK, a Regulator of Metabolism and Autophagy, Is Activated by Lysosomal..., ATG4 family proteins drive phagophore growth independently of the LC3/GA..., An optimized quantitative proteomics method establishes the cell type-re..., Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mit..., Autophagosome content profiling using proximity biotinylation proteomics..., Autophagy acts through TRAF3 and RELB to regulate gene expression via an..., Basic Fibroblast Growth Factor 2-Induced Proteome Changes Endorse Lewy B..., Beneficial Effect of ACI-24 Vaccination on Aβ Plaque Pathology and Micro..., Brain injury environment critically influences the connectivity of trans..., C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin c..., CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boos..., Cell-type-specific profiling of brain mitochondria reveals functional an..., Cellular depletion of major cathepsin proteases reveals their concerted ..., Deciphering sources of PET signals in the tumor microenvironment of glio..., Defining the Adult Neural Stem Cell Niche Proteome Identifies Key Regula..., Development of a Proteomic Workflow for the Identification of Heparan Su..., Distinct molecular profiles of skull bone marrow in health and neurologi..., Excessive local host-graft connectivity in aging and amyloid-loaded brain, Experimental evidence for temporal uncoupling of brain Aβ deposition and..., Fibrillar Aβ triggers microglial proteome alterations and dysfunction in..., Filling the Gaps – A Call for Comprehensive Analysis of Extracellular Ma..., IKKβ binds NLRP3 providing a shortcut to inflammasome activation for rap..., Injury-specific factors in the cerebrospinal fluid regulate astrocyte pl..., Lipid and protein content profiling of isolated native autophagic vesicles, Loss of CLN3 in microglia leads to impaired lipid metabolism and myelin ..., Loss of NPC1 enhances phagocytic uptake and impairs lipid trafficking in..., Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH, Lysosomal targeting of the ABC transporter TAPL is determined by membran..., Mapping autophagosome contents identifies interleukin-7 receptor-alpha a..., Met/HGFR triggers detrimental reactive microglia in TBI, MicroRNAs from extracellular vesicles as a signature for Parkinson's dis..., Multi-omics profiling identifies a deregulated FUS-MAP1B axis in ALS/FTD..., Multiomic ALS signatures highlight subclusters and sex differences sugge..., Neuronal differentiation of LUHMES cells induces substantial changes of ..., Nonvesicular lipid transfer drives myelin growth in the central nervous ..., NrCAM is a marker for substrate-selective activation of ADAM10 in Alzhei..., Pro-inflammatory activation following demyelination is required for myel..., Proteomic Characterization of Ubiquitin Carboxyl-Terminal Hydrolase 19 D..., Proteomic and lipidomic profiling of demyelinating lesions identifies fa..., Proteomic profiling in cerebral amyloid angiopathy reveals an overlap wi..., Proteomics of mouse brain endothelium uncovers dysregulation of vesicula..., Rational correction of pathogenic conformational defects in HTRA1, Reactivated endogenous retroviruses promote protein aggregate spreading, Signal peptide peptidase-like 2c impairs vesicular transport and cleaves..., Signatures of glial activity can be detected in the CSF proteome, Spatial centrosome proteome of human neural cells uncovers disease-relev..., Spatial proteomics in three-dimensional intact specimens, Spatial proteomics reveals secretory pathway disturbances caused by neur..., Systematically defining selective autophagy receptor-specific cargo usin..., Targeting the TCA cycle can ameliorate widespread axonal energy deficien..., The Alzheimer's disease-linked protease BACE1 modulates neuronal IL-6 si..., The Alzheimer's disease-linked protease BACE2 cleaves VEGFR3 and modulat..., The COP9 signalosome reduces neuroinflammation and attenuates ischemic n..., The Hippo network kinase STK38 contributes to protein homeostasis by inh..., The intramembrane protease SPPL2c promotes male germ cell development by..., The pseudoprotease iRhom1 controls ectodomain shedding of membrane prote..., The tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor com..., The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in respon..., Trnp1 organizes diverse nuclear membrane-less compartments in neural ste..., Ubiquitin profiling of lysophagy identifies actin stabilizer CNN2 as a t...
Assays: Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Affinity purification coupled with mass spectrometry proteomics (human), Bottom-up proteomics (human), Bottom-up proteomics (mouse), Bottom-up proteomics (mouse), Bottom-up proteomics (mouse), Bottom-up proteomics (mouse), Gel-based experiment (human), Phosphoproteomics / Bottom-up proteomics (mouse), Proximity-proteomics-based autophagosome content profiling (human), SWATH MS (human), SWATH MS (human, mouse), SWATH MS (mouse), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human), Shotgun proteomics (human, mouse), Shotgun proteomics (human, mouse), Shotgun proteomics (human, mouse), Shotgun proteomics (human, mouse), Shotgun proteomics (macaque), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (mouse), Shotgun proteomics (rat), Untargeted Proteomics (mouse)
Niemann-Pick type C (NPC) disease is a rare neurodegenerative disorder mainly caused by autosomal recessive mutations in Npc1 which result in abnormal late endosomal/lysosomal lipid storage. Although microgliosis is one of the prominent pathological features, consequences of NPC1 loss on microglial function and disease outcome remain largely unknown. Here, we provide an in-depth characterization of microglial proteomic signatures and phenotypes in an NPC1-deficient (Npc1-/-) murine model. We ...
Submitter: Rainer Malik
Investigation: Proteomics (Published)
Assays: Shotgun proteomics (human), Shotgun proteomics (mouse)
Primary microglia were isolated from mouse brains (cerebrum) using MACS Technology (Miltenyi Biotec) according to manufacturer´s instructions and as previously described (Daria et al., 2017). Briefly, brain cerebrum was dissected, freed from meninges and dissociated by enzymatic digestion using a Neural Tissue Dissociation Kit P (Miltenyi Biotec) and subsequent mechanical dissociation using 3 fire-polished glass Pasteur pipettes of decreasing diameter. CD11b positive microglia were magnetically ...
Submitter: Rainer Malik
Assay type: Proteomics
Technology type: Technology Type
Investigation: Proteomics (Published)
