Expression profiling by high throughput sequencing
SEEK ID: http://localhost:3000/assays/12
Experimental assay
Projects: SyNergy - published datasets
Investigation: Transcriptomics
Assay position:
Assay type: RNA-seq Profiling
Technology type: Technology Type
Organisms: No organisms
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Creators and SubmitterViews: 22
Created: 28th Jun 2024 at 08:50
Last updated: 28th Jun 2024 at 09:40
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Projects: SyNergy - published datasets
Institutions: Klinikum der Universität München
Public web page: Not specified
Submitter: Rainer Malik
Studies: A genome-wide in vivo CRISPR screen identifies essential regulators of T..., Correlation spatial transcriptomics/EM, Direct neuronal reprogramming of NDUFS4 patient cells identifies the unf..., Early adaptive immune activation detected in monozygotic twins with prod..., Histone Deacetylase 9 Activates IKK to Regulate Atherosclerotic Plaque V..., Innate immune memory after brain injury drives inflammatory cardiac dysf..., Microglia in white matter aging, Myelin degeneration in leucodystrophies, Oligodendrocytes in AD models, Oligodendrocytes in white matter aging, Phagocyte-mediated synapse removal in cortical neuroinflammation is prom..., Skin and gut imprinted helper T cell subsets exhibit distinct functional..., T cells modulate the microglial response to brain ischemia
Assays: Bulk RNA-seq, Expression profiling by high throughput sequencing, Expression profiling by high throughput sequencing, Expression profiling by high throughput sequencing, Expression profiling by high throughput sequencing, Expression profiling by high throughput sequencing, Expression profiling by high throughput sequencing, Expression profiling by high throughput sequencing, Expression profiling by high throughput sequencing, Expression profiling by high throughput sequencing, Expression profiling by high throughput sequencing, Single cell RNA sequencing experiment, scRNA-seq 5 day, scRNA-seq day 20
Submitter: Rainer Malik
Investigation: Transcriptomics
We developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant of T helper cell-induced immunopathology in remote organs. By site-specific in vivo labeling of antigen-specific T cells in inguinal (i) or gut draining mesenteric (m) lymph nodes, we show that i-T cells and m-T cells isolated from the inflamed central nervous system in a model for Multiple Sclerosis are distinct.
External LinkWe developed an experimental framework to probe the idea that the site of priming in the systemic immune compartment is a determinant of T helper cell-induced immunopathology in remote organs. By site-specific in vivo labeling of antigen-specific T cells in inguinal (i) or gut draining mesenteric (m) lymph nodes, we show that i-T cells and m-T cells isolated from the inflamed central nervous system in a model for Multiple Sclerosis are distinct.
Single-cell transcriptome profiling of disease-related lymphocytes in patients with multiple sclerosis
Here, we use in vivo labeling at defined anatomical sites for "provance" tracking of immune cells across compartmental borders in the context of experimental autoimmune encephalomyelitis (EAE). Specifically, we labeled T cells in mesenteric or inguinal lymph nodes (LNs) of T cell conditional mitoDendra2 reporter mice via photoconversion at disease onset and re-isolated photoconverted T cells form the LNs, spleen and CNS two days later and we performed scRNA-seq on them. Our experimental system ...
