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Organisms: Mus musculus, Rattus norvegicus, Homo sapiens, Macaca mulatta, Sus scrofa
Microglial dysfunction is a key pathological feature of Alzheimer´s disease (AD), but little is known about proteome-wide changes in microglia during the course of AD pathogenesis and their consequences for microglial function. Here, we performed an in-depth proteomic characterization of microglia in two AD mouse models, the overexpression APPPS1 and the knock-in AppNL-G-F (APP-KI) model. Proteome changes were followed from pre-deposition to early, middle and advanced stages of amyloid plaque ...
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Here, we took advantage of well-defined mouse models for β-amyloidosis (APPPS1) to explore proteome changes in the cerebrospinal fluid which are related to these distinct proteopathic lesions. Non-targeted liquid chromatography-mass spectrometry revealed that the majority of proteins that undergo age- and disease-related changes in either mouse model was linked to microglia, and more specifically to previously described disease state-specific microglia transcriptomic signatures. The finding that ...
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Submitter: Rainer Malik
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Proteomics
Organisms: Mus musculus
SOPs: No SOPs
Data files: Cerebrospinal Fluid (CSF) Proteomics of A30P-αS..., Cerebrospinal Fluid (CSF) Proteomics of APPPS1 ...
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Primary microglia were isolated from mouse brains (cerebrum) using MACS Technology (Miltenyi Biotec) according to manufacturer´s instructions and as previously described (Daria et al., 2017). Briefly, brain cerebrum was dissected, freed from meninges and dissociated by enzymatic digestion using a Neural Tissue Dissociation Kit P (Miltenyi Biotec) and subsequent mechanical dissociation using 3 fire-polished glass Pasteur pipettes of decreasing diameter. CD11b positive microglia were magnetically ...
Submitter: Rainer Malik
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Proteomics
Organisms: Mus musculus
SOPs: No SOPs
Data files: Microglial proteomic signatures in APPPS1 and A...
Snapshots: No snapshots
Microglial dysfunction is a key pathological feature of Alzheimer´s disease (AD), but little is known about proteome-wide changes in microglia during the course of AD pathogenesis and their consequences for microglial function. Here, we performed an in-depth proteomic characterization of microglia in two AD mouse models, the overexpression APPPS1 and the knock-in AppNL-G-F (APP-KI) model. Proteome changes were followed from pre-deposition to early, middle and advanced stages of amyloid plaque ...
Creators: Rainer Malik, Stephan Müller, Stefan Lichtenthaler, Jochen Herms, Arthur Liesz, Christian Haass
Submitter: Rainer Malik
Investigations: Proteomics
Studies: Fibrillar Aβ triggers microglial proteome alter...
Assays: Shotgun proteomics
Here, we took advantage of well-defined mouse models for α-synucleinopathy (A30P-αS ) to explore proteome changes in the cerebrospinal fluid which are related to these distinct proteopathic lesions. Non-targeted liquid chromatography-mass spectrometry revealed that the majority of proteins that undergo age- and disease-related changes in either mouse model was linked to microglia, and more specifically to previously described disease state-specific microglia transcriptomic signatures. The finding ...
Creators: Rainer Malik, Stefan Lichtenthaler, Stephan Müller, Christian Haass
Submitter: Rainer Malik
Investigations: Proteomics
Studies: Signatures of glial activity can be detected in...
Assays: Shotgun proteomics
Here, we took advantage of well-defined mouse models for β-amyloidosis (APPPS1) to explore proteome changes in the cerebrospinal fluid which are related to these distinct proteopathic lesions. Non-targeted liquid chromatography-mass spectrometry revealed that the majority of proteins that undergo age- and disease-related changes in either mouse model was linked to microglia, and more specifically to previously described disease state-specific microglia transcriptomic signatures. The finding that ...
Creators: Rainer Malik, Stefan Lichtenthaler, Stephan Müller, Christian Haass
Submitter: Rainer Malik
Investigations: Proteomics
Studies: Signatures of glial activity can be detected in...
Assays: Shotgun proteomics
Abstract (Expand)
Authors: T. Eninger, S. A. Muller, M. Bacioglu, M. Schweighauser, M. Lambert, L. F. Maia, J. J. Neher, S. M. Hornfeck, U. Obermuller, G. Kleinberger, C. Haass, P. J. Kahle, M. Staufenbiel, L. Ping, D. M. Duong, A. I. Levey, N. T. Seyfried, S. F. Lichtenthaler, M. Jucker, S. A. Kaeser
Date Published: 14th Jun 2022
Publication Type: Journal
PubMed ID: 35666874
Citation: Proc Natl Acad Sci U S A. 2022 Jun 14;119(24):e2119804119. doi: 10.1073/pnas.2119804119. Epub 2022 Jun 6.
Abstract (Expand)
Authors: L. Sebastian Monasor, S. A. Muller, A. V. Colombo, G. Tanrioever, J. Konig, S. Roth, A. Liesz, A. Berghofer, A. Piechotta, M. Prestel, T. Saito, T. C. Saido, J. Herms, M. Willem, C. Haass, S. F. Lichtenthaler, S. Tahirovic
Date Published: 8th Jun 2020
Publication Type: Journal
PubMed ID: 32510331
Citation: Elife. 2020 Jun 8;9:e54083. doi: 10.7554/eLife.54083.