Expertise: Not specified
Tools: Not specified
Related items
Public web page: Not specified
Organisms: Mus musculus, Rattus norvegicus, Homo sapiens, Macaca mulatta, Sus scrofa
Microglial dysfunction is a key pathological feature of Alzheimer´s disease (AD), but little is known about proteome-wide changes in microglia during the course of AD pathogenesis and their consequences for microglial function. Here, we performed an in-depth proteomic characterization of microglia in two AD mouse models, the overexpression APPPS1 and the knock-in AppNL-G-F (APP-KI) model. Proteome changes were followed from pre-deposition to early, middle and advanced stages of amyloid plaque ...
Snapshots: No snapshots
Submitter: Rainer Malik
Investigation: Transcriptomics
Snapshots: No snapshots
Snapshots: No snapshots
Primary microglia were isolated from mouse brains (cerebrum) using MACS Technology (Miltenyi Biotec) according to manufacturer´s instructions and as previously described (Daria et al., 2017). Briefly, brain cerebrum was dissected, freed from meninges and dissociated by enzymatic digestion using a Neural Tissue Dissociation Kit P (Miltenyi Biotec) and subsequent mechanical dissociation using 3 fire-polished glass Pasteur pipettes of decreasing diameter. CD11b positive microglia were magnetically ...
Submitter: Rainer Malik
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Proteomics
Organisms: Mus musculus
SOPs: No SOPs
Data files: Microglial proteomic signatures in APPPS1 and A...
Snapshots: No snapshots
Microglial dysfunction is a key pathological feature of Alzheimer´s disease (AD), but little is known about proteome-wide changes in microglia during the course of AD pathogenesis and their consequences for microglial function. Here, we performed an in-depth proteomic characterization of microglia in two AD mouse models, the overexpression APPPS1 and the knock-in AppNL-G-F (APP-KI) model. Proteome changes were followed from pre-deposition to early, middle and advanced stages of amyloid plaque ...
Creators: Rainer Malik, Stephan Müller, Stefan Lichtenthaler, Jochen Herms, Arthur Liesz, Christian Haass
Submitter: Rainer Malik
Investigations: Proteomics
Studies: Fibrillar Aβ triggers microglial proteome alter...
Assays: Shotgun proteomics
The crosstalk between brain infiltrating T cells and microglia in response to stroke remains elusive. Benakis et al. report that transcriptional signature of the stroke-associated microglia is reprogrammed by distinct T cell subpopulations. Engineered T cells overexpressing IL-10 administered four hours after stroke reinitiate microglial transcriptomic profile inducing a pro-regenerative environment.
Creator: Arthur Liesz
Submitter: Rainer Malik
Investigations: Transcriptomics
Abstract (Expand)
Authors: C. Benakis, A. Simats, S. Tritschler, S. Heindl, S. Besson-Girard, G. Llovera, K. Pinkham, A. Kolz, A. Ricci, F. J. Theis, S. Bittner, O. Gokce, A. Peters, A. Liesz
Date Published: 13th Dec 2022
Publication Type: Journal
PubMed ID: 36512388
Citation: Elife. 2022 Dec 13;11:e82031. doi: 10.7554/eLife.82031.
Abstract (Expand)
Authors: T. Kaya, N. Mattugini, L. Liu, H. Ji, L. Cantuti-Castelvetri, J. Wu, M. Schifferer, J. Groh, R. Martini, S. Besson-Girard, S. Kaji, A. Liesz, O. Gokce, M. Simons
Date Published: 26th Oct 2022
Publication Type: Journal
PubMed ID: 36280798
Citation: Nat Neurosci. 2022 Nov;25(11):1446-1457. doi: 10.1038/s41593-022-01183-6. Epub 2022 Oct 24.
Abstract (Expand)
Authors: L. Sebastian Monasor, S. A. Muller, A. V. Colombo, G. Tanrioever, J. Konig, S. Roth, A. Liesz, A. Berghofer, A. Piechotta, M. Prestel, T. Saito, T. C. Saido, J. Herms, M. Willem, C. Haass, S. F. Lichtenthaler, S. Tahirovic
Date Published: 8th Jun 2020
Publication Type: Journal
PubMed ID: 32510331
Citation: Elife. 2020 Jun 8;9:e54083. doi: 10.7554/eLife.54083.