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Organisms: Mus musculus, Rattus norvegicus, Homo sapiens, Macaca mulatta, Sus scrofa
Mitochondria vary in morphology and function in different tissues, however little is known about their molecular diversity among cell types. To investigate mitochondrial diversity in vivo, we developed an efficient protocol to isolate cell type-specific mitochondria based on a new MitoTag mouse. We profiled the mitochondrial proteome of three major neural cell types in cerebellum and identified a substantial number of differential mitochondrial markers for these cell types in mice and humans. ...
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Samples for mass spectrometry were obtained from 8- to 9-week-old, male mice. Mitochondria were immunopurified from cerebellum according to the described protocol with the alteration that the final mitochondrial pellet was washed twice in IB without EDTA and BSA. Sample were lysed in 100 µL SDT lysis buffer (4% w:v SDS, 100 mM DTT in 100 mM Tris-HCl pH 7.6) by heating for 5 min at 95°C and ultrasonication (Vialtweeter: 6 times for 30 s, 100% Amplitude, 50% cycle, max power; Hielscher Ultrasonics). ...
Submitter: Rainer Malik
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Proteomics
Organisms: Mus musculus
SOPs: No SOPs
Data files: Quantitative proteomics of immunocaptured mitoc..., Quantitative proteomics of immunocaptured mitoc..., Quantitative proteomics of immunocaptured mitoc...
Snapshots: No snapshots
Mitochondria vary in morphology and function in different tissues, however little is known about their molecular diversity among cell types. To investigate mitochondrial diversity in vivo, we developed an efficient protocol to isolate cell type-specific mitochondria based on a new MitoTag mouse. We profiled the mitochondrial proteome of three major neural cell types in cerebellum and identified a substantial number of differential mitochondrial markers for these cell types in mice and humans. ...
Creators: Rainer Malik, Stefan Lichtenthaler, Stephan Müller, Thomas Misgeld, Arthur Konnerth, Thomas Korn
Submitter: Rainer Malik
Investigations: Proteomics
Studies: Cell-type-specific profiling of brain mitochond...
Assays: Shotgun proteomics
Mitochondria vary in morphology and function in different tissues, however little is known about their molecular diversity among cell types. To investigate mitochondrial diversity in vivo, we developed an efficient protocol to isolate cell type-specific mitochondria based on a new MitoTag mouse. We profiled the mitochondrial proteome of three major neural cell types in cerebellum and identified a substantial number of differential mitochondrial markers for these cell types in mice and humans. ...
Creators: Rainer Malik, Stephan Müller, Thomas Misgeld, Stefan Lichtenthaler, Arthur Konnerth, Thomas Korn
Submitter: Rainer Malik
Investigations: Proteomics
Studies: Cell-type-specific profiling of brain mitochond...
Assays: Shotgun proteomics
Mitochondria vary in morphology and function in different tissues, however little is known about their molecular diversity among cell types. To investigate mitochondrial diversity in vivo, we developed an efficient protocol to isolate cell type-specific mitochondria based on a new MitoTag mouse. We profiled the mitochondrial proteome of three major neural cell types in cerebellum and identified a substantial number of differential mitochondrial markers for these cell types in mice and humans. ...
Creators: Rainer Malik, Stephan Müller, Stefan Lichtenthaler, Thomas Misgeld, Arthur Konnerth, Thomas Korn
Submitter: Rainer Malik
Investigations: Proteomics
Studies: Cell-type-specific profiling of brain mitochond...
Assays: Shotgun proteomics
Abstract (Expand)
Authors: M. Hiltensperger, E. Beltran, R. Kant, S. Tyystjarvi, G. Lepennetier, H. Dominguez Moreno, I. J. Bauer, S. Grassmann, S. Jarosch, K. Schober, V. R. Buchholz, S. Kenet, C. Gasperi, R. Ollinger, R. Rad, A. Muschaweckh, C. Sie, L. Aly, B. Knier, G. Garg, A. M. Afzali, L. A. Gerdes, T. Kumpfel, S. Franzenburg, N. Kawakami, B. Hemmer, D. H. Busch, T. Misgeld, K. Dornmair, T. Korn
Date Published: 9th Jun 2021
Publication Type: Journal
PubMed ID: 34099917
Citation: Nat Immunol. 2021 Jul;22(7):880-892. doi: 10.1038/s41590-021-00948-8. Epub 2021 Jun 7.
Abstract (Expand)
Authors: C. Fecher, L. Trovo, S. A. Muller, N. Snaidero, J. Wettmarshausen, S. Heink, O. Ortiz, I. Wagner, R. Kuhn, J. Hartmann, R. M. Karl, A. Konnerth, T. Korn, W. Wurst, D. Merkler, S. F. Lichtenthaler, F. Perocchi, T. Misgeld
Date Published: 11th Sep 2019
Publication Type: Journal
PubMed ID: 31501572
Citation: Nat Neurosci. 2019 Oct;22(10):1731-1742. doi: 10.1038/s41593-019-0479-z. Epub 2019 Sep 9.