Assays
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Created At
Updated At
Project
SyNergy - published datasets1
Submitter
Rainer Malik1
Creator
Rainer Malik1
Stephan Müller1
Stefan Lichtenthaler1
Arthur Konnerth1
Thomas Korn1
Thomas Misgeld1
Organism
Mus musculus1
Assay class
Experimental assay1
Assay type
Experimental Assay Type1
Technology type
Technology Type1
1
Assay visible to you, out of a total of 1
Samples for mass spectrometry were obtained from 8- to 9-week-old, male mice. Mitochondria were immunopurified from cerebellum according to the described protocol with the alteration that the final mitochondrial pellet was washed twice in IB without EDTA and BSA. Sample were lysed in 100 µL SDT lysis buffer (4% w:v SDS, 100 mM DTT in 100 mM Tris-HCl pH 7.6) by heating for 5 min at 95°C and ultrasonication (Vialtweeter: 6 times for 30 s, 100% Amplitude, 50% cycle, max power; Hielscher Ultrasonics). ...
Submitter: Rainer Malik
Assay type: Experimental Assay Type
Technology type: Technology Type
Investigation: Proteomics