Signal peptide peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins.

Abstract:

Members of the GxGD-type intramembrane aspartyl proteases have emerged as key players not only in fundamental cellular processes such as B-cell development or protein glycosylation, but also in development of pathologies, such as Alzheimer's disease or hepatitis virus infections. However, one member of this protease family, signal peptide peptidase-like 2c (SPPL2c), remains orphan and its capability of proteolysis as well as its physiological function is still enigmatic. Here, we demonstrate that SPPL2c is catalytically active and identify a variety of SPPL2c candidate substrates using proteomics. The majority of the SPPL2c candidate substrates cluster to the biological process of vesicular trafficking. Analysis of selected SNARE proteins reveals proteolytic processing by SPPL2c that impairs vesicular transport and causes retention of cargo proteins in the endoplasmic reticulum. As a consequence, the integrity of subcellular compartments, in particular the Golgi, is disturbed. Together with a strikingly high physiological SPPL2c expression in testis, our data suggest involvement of SPPL2c in acrosome formation during spermatogenesis.

SEEK ID: http://lmmeisd-2.srv.mwn.de/publications/32

PubMed ID: 30733281

Projects: Published Datasets

Publication type: Journal

Journal: EMBO Rep

Citation: EMBO Rep. 2019 Mar;20(3):e46451. doi: 10.15252/embr.201846451. Epub 2019 Feb 7.

Date Published: 9th Feb 2019

Registered Mode: by PubMed ID

Authors: A. A. Papadopoulou, S. A. Muller, T. Mentrup, M. D. Shmueli, J. Niemeyer, M. Haug-Kroper, J. von Blume, A. Mayerhofer, R. Feederle, B. Schroder, S. F. Lichtenthaler, R. Fluhrer

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Created: 5th Jul 2024 at 09:37

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