Studies

The centrosome acts as the cell’s microtubule organizing center, supporting cell division and the extension of cilia and neurites. Newly born neurons require the microtubule organizing activity of centrosomes to migrate away from their birthplace at the ventricle. O’Neill et al. analyzed the centrosome proteome of human induced pluripotent stem cell–derived neural stem cells and neurons. The neural centrosome proteome contains a variety of RNA-binding/modifying proteins, including an RNA-splicing ...

The centrosome provides an intracellular anchor for the cytoskeleton, regulating cell division, cell migration, and cilia formation. We used spatial proteomics to elucidate protein interaction networks at the centrosome of human induced pluripotent stem cell-derived neural stem cells (NSCs) and neurons. Centrosome-associated proteins were largely cell type-specific, with protein hubs involved in RNA dynamics. Analysis of neurodevelopmental disease cohorts identified a significant overrepresentation ...

TMF1-regulated nuclear protein 1 (Trnp1) has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding, but its molecular function in the nucleus is still unknown. Here, we show that Trnp1 is a low complexity protein with the capacity to phase separate. Trnp1 interacts with factors located in several nuclear membrane-less organelles, the nucleolus, nuclear speckles, and condensed chromatin. Importantly, Trnp1 co-regulates the architecture and ...