Data files

RNA from FACS sorted electroporated (GFP+) cells was isolated in Extraction Buffer (Arcturus), heated to 42 and stored at -80 cDNA was synthesized from 300 pg of total RNA using SMART‐Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech), according to the manufacturer's instructions. Prior to generating the final library for Illumina sequencing, the Covaris AFA system was used to perform the cDNA shearing, resulting in 200‐ to 500‐bp‐long cDNA fragments. The quality and concentration of the ...

The crosstalk between brain infiltrating T cells and microglia in response to stroke remains elusive. Benakis et al. report that transcriptional signature of the stroke-associated microglia is reprogrammed by distinct T cell subpopulations. Engineered T cells overexpressing IL-10 administered four hours after stroke reinitiate microglial transcriptomic profile inducing a pro-regenerative environment. CD45+CD11b+ cells sorted from the ipsilateral hemisphere in naïve mice or 5 d after stroke in WT ...

CUT&tag sequencing of mouse BM monocytes and HSPC-enriched myeloid cells from mice one month after stroke or control conditions was performed

Bulk mRNA sequencing of human heart paraffin embeded samples from stroke patients and control patients

Bulk ATAC sequencing of mouse HSPC-enriched BM cells treated with recombinant IL-1b or vehicle

Mouse single-nuclei ATAC experiment performed on myeloid cells from bone marrow chronically after stroke and control conditions. An additional group of bone marrow cells from stroke mice treated with neutralizing antibodies against IL-1b was also included (stroke-treated group).

A single-cell RNA experiment was performed on cardiac interstitial cells from mice one month after stroke or control conditions