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2 Data files visible to you, out of a total of 2

Effect of CCK-8s injection (20 ug/kg BW i.p.) on mRNA translational activity in hypothalamic oxytocin neurons of mice fed standard chow or high-fat/high-sugar diet (RiboTag;TRAP)

Published Datasets

We treated male Oxt-ires-Cre;RiboTag mice with either vehicle or CCK, collected hypothalami 2h post injection, pooled 2-3 tissues per sample, and affinity purified conditionally HA-tagged ribosomes (incl translating mRNA) specifically from oxytocin neurons. We then performed gene expression profiling analysis using data obtained from RNA-seq of immunoprecipitates versus inputs (n=4 per group) from standard chow diet fed mice and inputs only from high-fat/high-sugar diet fed mice (n=4 per group). ...

Creators: None

Submitter: Aditi Methi

Investigations: Transcriptomics (Published)

Studies: High-calorie diets uncouple hypothalamic oxytoc...

Assays: Expression profiling: RiboTag-mRNA-seq (mouse)

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Created: 2nd Dec 2024 at 13:29

Gene expression profiles at the single nuclei level of hypothalamic oxytocin neurons from mice fed either standard chow (SC) diet or high-fat/high-sugar (HFHS) diet

Published Datasets

Hypothalamic oxytocin neurons from Oxt-ires-Cre;CAG-Sun1sfGFP mice were isolated by Fluorescence-activated nuclei sorting (FANS) according to the presence or absence of sfGFP signal and analyzed using snRNAseq2.