Data files
What is a Data file?Filters
Query
Created At
Updated At
Submitter
Aditi Methi1
Project
SyNergy - Published Datasets1
Assay type
Transcriptomics1
Technology type
Sequencing1
1
Data file visible to you, out of a total of 1
RNA from FACS sorted electroporated (GFP+) cells was isolated in Extraction Buffer (Arcturus), heated to 42 and stored at -80 cDNA was synthesized from 300 pg of total RNA using SMART‐Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech), according to the manufacturer's instructions. Prior to generating the final library for Illumina sequencing, the Covaris AFA system was used to perform the cDNA shearing, resulting in 200‐ to 500‐bp‐long cDNA fragments. The quality and concentration of the ...
Creators: None
Submitter: Aditi Methi