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Creators: Rainer Malik, Christian Behrends
Submitter: Rainer Malik
The human lysosomal polypeptide ABC transporter TAPL (ABC subfamily B member 9, ABCB9) transports 6–59 amino-acids-long polypeptides from the cytosol into lysosomes. The subcellular localization of TAPL depends solely on its N-terminal transmembrane domain TMD0, which lacks conventional targeting sequences. However, the intracellular route and the molecular mechanisms that control TAPL localization remain unclear. Here, we delineated the route of TAPL to lysosomes and investigated the determinants ...
Creators: Rainer Malik, Christian Behrends
Submitter: Rainer Malik
HEK293T cells stably expressing N-terminally HA-tagged BAG3 were employed to screen for novel BAG3 interacting proteins.
Creators: Rainer Malik, Christian Behrends
Submitter: Rainer Malik
SILAC-based proximity proteomics of UAPEX2-tagged UBE2QL1 in differential LLOMe-treated HeLa cells
Creators: Rainer Malik, Christian Behrends
Submitter: Rainer Malik
We selected GABARAPL2endoHA cells for a proof-of-principle immunoprecipitation (IP) followed by mass spectrometric (MS) analysis to identify new binding partner candidates of a hATG8 family member at endogenous levels. To distinguish between candidates that bind preferentially to PE-conjugated versus unconjugated GABARAPL2 we treated stable isotope labeling with amino acids in cell culture (SILAC)-labeled GABARAPL2endoHA cells with Torin1 and BafA1 (light) or ATG7 inhibitor (heavy).
Creators: Rainer Malik, Christian Behrends
Submitter: Rainer Malik
Proximity proteomics of GAL9 upon lysosomal damage by LLOMe or GPN. Proximity labeling was performed in SILAC labelled HEK293T cells stably expressing APEX2-GAL9.
Creators: Rainer Malik, Christian Behrends
Submitter: Rainer Malik
Identification of autophagy substrates by directing APEX2 to autophagosomes and immune isolation of lysosomes.
Creators: Rainer Malik, Christian Behrends
Submitter: Rainer Malik