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To identify ubiquitylation targets following lysosomal damage in HeLa cells treated with LLOMe we performed quantitative ubiquitin-remmnant (diGly) profilig coupled to mass spectrometry (MS). In addition, we performed APEX2-based proximity biotinylation followed by MS analysis to identify proximity partners of one of the ubiquitylation targets (CNN2).
Submitter: Rainer Malik
Investigation: Proteomics
Assays: Affinity purification coupled with mass spectrometry proteomics, Shotgun proteomics