Data files

Purpose of the study is to compare the transcriptional profiles of astrocytes obtained from cortex gray matter and spinal cord of postnatal mice. Astrocytes were isolated from 2 regions via ACSA-2 sorting, cultured for 7 days and then subjected to RNA-seq

Spinal cord-derived astrocytes were transduced in cultures with retrovirus enconding a tamoxifen-dependent form of Ascl1 and Neurog2.Cells were treated for 24 hours, then transduced cells were FACS-sorted and their transcriptome analyzed.