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2 Data files visible to you, out of a total of 2

Gene expression profile at single cell level of cells derived from the arcuate nucleus of the hypothalamus (ARC) of mice fed with standard chow (SC) or high-fat high-sugar (HFHS) diet

Published Datasets

ARC tissue derived from C57BL/6JRj mice fed with SC diet (control), 5 days of HFHS (58%) diet, and 15 days of HFHS (58%) diet was dissociated by enzymatic digestion into single cells, which were then analyzed by scRNA-Seq. Per each experimental group, the ARCs from 6 animals were pulled into one sample.

Creators: None

Submitter: Aditi Methi

Investigations: Transcriptomics (Published)

Studies: Diet triggers specific responses of hypothalami...

Assays: Expression profiling: scRNA-seq (mouse)

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Created: 2nd Dec 2024 at 13:19

Gene expression profile of astrocytes derived from the cortex, hippocampus and hypothalamus of mice fed with standard chow (SC) or high-fat high-sugar (HFHS) diet

Published Datasets

C57BL/6JRj mice were exposed to a SC or a HFHS (58%) diet at 8-9 weeks of age for 4 months. Afterwards, hypothalami, hippocampi, and half cortices derived from 2 animals were isolated and pooled together in distinct tubes. A number of 5-7 replicates per each group was used. ACSA2+ astrocytes were isolated from each sample by magnetic-activated cell sorting (MACS), and further processed for RNA-Sequencing analysis.