Data files

After respective treatment, cells were washed 2 times with ice-cold DPBS, scraped in PBS and pellets either processed immediately or stored at -80°C. Cells were lysed in RIPA buffer for 30 min on ice. After clearance by centrifugation at 20,000 × g for 10 min at 4 °C, protein concentrations were adjusted using BCA assay. Cleared and adjusted supernatants were incubated overnight on an overhead rotator with preequilibrated streptavidin agarose (Sigma). Next day, beads were washed 2 times with RIPA ...