Assays
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Project
Published Datasets1
Submitter
Aditi Methi1
Organism
Homo sapiens1
Assay class
Experimental assay1
Assay type
Proteomics1
Technology type
Technology Type1
For proteomic analysis, iPSC-derived neural stem cells at day 15 of culture were treated with 3.3 uM Nocodazole (Sigma) or same volume of DMSO (control) before being washed with ice-cold PBS, scrapped and centrifuged for 10 min at 300 g before lysis in Buffer A, containing 50 mM Tris-HCL (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton-X100, 0.1% SDS, protease inhibitor (Pierce), at a volume twice that of the pellet. For neurons, day 40 of cultures treated only with DMSO were used. Samples ...
Submitter: Aditi Methi
Assay type: Proteomics
Technology type: Technology Type
Investigation: Proteomics (Published)