Data files

MACS-sorted astrocytes, obtained from postnatal mouse spinal cord, were transduced in vitro with Ascl1 and Neurog2. Reprogrammed cells were collected from the coverslips by suction and subjected to SmartSeq2 amplification

Purpose of the study is to compare the transcriptional profiles of astrocytes obtained from cortex gray matter and spinal cord of postnatal mice. Astrocytes were isolated from 2 regions via ACSA-2 sorting, cultured for 7 days and then subjected to RNA-seq

Spinal cord-derived astrocytes were transduced in cultures with retrovirus enconding a tamoxifen-dependent form of Ascl1 and Neurog2.Cells were treated for 24 hours, then transduced cells were FACS-sorted and their transcriptome analyzed.

RNA from FACS sorted electroporated (GFP+) cells was isolated in Extraction Buffer (Arcturus), heated to 42 and stored at -80 cDNA was synthesized from 300 pg of total RNA using SMART‐Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech), according to the manufacturer's instructions. Prior to generating the final library for Illumina sequencing, the Covaris AFA system was used to perform the cDNA shearing, resulting in 200‐ to 500‐bp‐long cDNA fragments. The quality and concentration of the ...

CUT&tag sequencing of mouse BM monocytes and HSPC-enriched myeloid cells from mice one month after stroke or control conditions was performed

Bulk mRNA sequencing of human heart paraffin embeded samples from stroke patients and control patients

Bulk ATAC sequencing of mouse HSPC-enriched BM cells treated with recombinant IL-1b or vehicle